Process for the separation of double-stranded/single-stranded nucleic acid structures

The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded an...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Bastian, Helge, Gauch, Simone, Colpan, Metin, Feuser, Petra
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.