Method and apparatus for performing multiple processing steps on a sample in a single vessel

A method is provided for determining crystallization conditions for a protein, the method comprising: performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 50...

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Bibliographische Detailangaben
1. Verfasser: Downs, Robert Charles
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method is provided for determining crystallization conditions for a protein, the method comprising: performing in a same fermentation apparatus 2 or more separate fermentations where each fermentation comprises cells expressing a protein to be purified and has a fermentation volume of less than 500 mL; purifying the expressed protein from the separate fermentations; and determining crystallization conditions for the purified protein by submicroliter crystallization experiments.