Apparatus and method for the preparation of experiments using proteins contained within gels

An apparatus and method for dispensing protein-containing materials, such as, for example, pastes, gels, lipidic cubic phases, lipidic mesophases, and mixtures of the foregoing into containers, e.g., wells of assay trays. The invention further provides a method for preparing the aforementioned conta...

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Bibliographische Detailangaben
Hauptverfasser: Olson, Jeffrey A, Chiu, Mark L, Pan, Jeffrey Y, McCoy, Michael D
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:An apparatus and method for dispensing protein-containing materials, such as, for example, pastes, gels, lipidic cubic phases, lipidic mesophases, and mixtures of the foregoing into containers, e.g., wells of assay trays. The invention further provides a method for preparing the aforementioned containers for various experiments involving soluble proteins or membrane proteins or both types of proteins. In a preferred embodiment, the apparatus includes (a) a gel dispenser assembly for dispensing small quantities of gels; (b) a pipette probe for dispensing fluids; (c) an actuator for moving the gel dispenser assembly vertically relative to the pipette probe; (d) a liquid-handling robot having a robot arm for moving the pipette probe and the gel dispenser assembly vertically and horizontally relative to containers, e.g., wells of assay trays; (e) at least one pump for delivering a substantially incompressible fluid to the gel dispenser assembly; and (f) at least one pump for delivering reagents, and, optionally, other materials, to the pipette probe.