Method of in vitro differentiation of transplantable neural precursor cells from primate embryonic stem cells

A method of differentiating embryonic stem cells into neural precursor cells is disclosed. In one embodiment the method comprises the steps of (a) obtaining an embryonic stem cell culture, (b) propagating the stem cells, (c) forming embryoid bodies from the stem cells, and (d) culturing the embryoid...

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Bibliographische Detailangaben
Hauptverfasser: Zhang, Su-Chun, Thomson, James A, Duncan, Ian David
Format: Patent
Sprache:eng
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Zusammenfassung:A method of differentiating embryonic stem cells into neural precursor cells is disclosed. In one embodiment the method comprises the steps of (a) obtaining an embryonic stem cell culture, (b) propagating the stem cells, (c) forming embryoid bodies from the stem cells, and (d) culturing the embryoid bodies in a medium containing an effective amount of fibroblast growth factor 2, wherein neural precursor cells will be generated and isolated.