Methods for quantitative and qualitative analyses of phospholipids using one-dimensional thin layer chromatography

The present invention relates to methods for quantitative and qualitative analyses of phospholipids. Specifically, the present invention provides methods of using one-dimensional thin layer chromatography to detect and quantify phospholipids. More specifically, the present invention provides one-dimensional thin layer chromatography methods used to detect and quantify the phospholipid content of mammalian tissues. A highly sensitive and specific method for the detection and quantification of lipids is provided. Specifically, methods for the simultaneous detection and quantification of phospholipids extracted from mammalian tissues is described. The analytical methods provided disclose a modified one-dimensional thin-layer chromatography technique specifically developed to rapidly and accurantely detect and quantify phospholipids from mammalian cardiac tissues.