Electrophoresis gels

This invention relates to the field of gel electrophoresis, particularly to pre-cast polyacrylamide gels having an extended shelf-life. A polyacrylamide gel utilizing a buffer system comprising Tris(hydroxymethyl)aminomethane at the concentration range 0.15 to 0.25 M titrated with hydrochloric acid...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Chan, Grace Yim Ngan, Boyd, Nicola Sarah Frances, Gooley, Sue Ann, Solomon, David Henry
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:This invention relates to the field of gel electrophoresis, particularly to pre-cast polyacrylamide gels having an extended shelf-life. A polyacrylamide gel utilizing a buffer system comprising Tris(hydroxymethyl)aminomethane at the concentration range 0.15 to 0.25 M titrated with hydrochloric acid to a pH between 6.5 and 7.5. The gel is substantially stable to polyacrylamide hydrolysis during storage for at extended periods and has an acceptable shelf-life of at least 6 months after storage at about 4° C.