Amplification-based cloning method

The invention concerns a method for the enrichment and isolation of genes of interest from recombinant DNA libraries. The invention provides a method for isolating a gene of interest from a cDNA library, which involves the use of an amplification step to amplify the gene of interest and an enrichmen...

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Bibliographische Detailangaben
Hauptverfasser: Chui, Clarissa J, Grimaldi, J. Christopher, Milton, Sean, Yan, Minhong, Yi, Sothy
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention concerns a method for the enrichment and isolation of genes of interest from recombinant DNA libraries. The invention provides a method for isolating a gene of interest from a cDNA library, which involves the use of an amplification step to amplify the gene of interest and an enrichment step to remove the template plasmids.