Multi-well filtration apparatus

The present invention generally relates to filtration apparatus and more specifically relates to a multi-well filtration or solid phase extraction apparatus. A multi-well filtration or solid phase extraction apparatus is provided which generally includes a vacuum collar adapted to be interconnected between a conventional 96-well sample application plate, or separation plate, and a 96-well collection plate. The vacuum collar provides an interface chamber between these plates through which a vacuum can be applied to assist in the filtration process. The collection plate may include a vent opening providing a direct communication between the interface chamber and a vacuum assembly air intake, when the collection plate is disposed on top of the vacuum assembly in a stacked configuration, thereby eliminating any need for separate vacuum manifolds or connectors.