Apparatus and methods for arraying solution onto a solid support

This invention relates to microfabrication technology, such as DNA chip-making technology, and more specifically to methods and apparatuses for delivering controlled amounts of a solution to specific, closely spaced locations on a solid support. A method for depositing biomolecule onto a solid suppo...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Moynihan, Kristen, Ness, Jeffrey Van, Tabone, John C
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:This invention relates to microfabrication technology, such as DNA chip-making technology, and more specifically to methods and apparatuses for delivering controlled amounts of a solution to specific, closely spaced locations on a solid support. A method for depositing biomolecule onto a solid support, the method including the steps of: immersing a tip of a spring probe into a solution of biomolecule; removing said tip from said solution to provide biomolecule solution adhered to said tip; and contacting said biomolecule solution with a solid support to thereby transfer biomolecule solution from said tip to said solid support. The spring probe has a planar tip but it otherwise identical to commercial spring probes. The solution of biomolecule contains a thickening agent in addition to biomolecule, where oligonucleotide is a preferred biomolecule.