Apparatus for collecting nucleic acid and method of collecting nucleic acid

Provided herein are an apparatus and a method for efficiently recovering nucleic acids from a nucleic acid-containing solution by causing the nucleic acid-containing solution to come into stable contact with a solid phase in a nucleic acid-capturing tip. Recovery of nucleic acids is accomplished by...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sakurai, Toshinari, Yokobayashi, Toshiaki
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Provided herein are an apparatus and a method for efficiently recovering nucleic acids from a nucleic acid-containing solution by causing the nucleic acid-containing solution to come into stable contact with a solid phase in a nucleic acid-capturing tip. Recovery of nucleic acids is accomplished by aspirating a solution containing nucleic acids into a nucleic acid-capturing tip having a solid phase capable of capturing nucleic acids and discharging the aspirated solution, thereby causing said solid phase to capture the nucleic acids contained in the sample, with the rate of aspiration and discharging being controlled so as to increase the ratio of Nr/Ns, where Ns is the concentration of nucleic acids contained in the solution before aspiration and Nr is the concentration of nucleic acids contained in the solution after discharging.