Methods of detecting CD34 positive and negative hematopoietic stem cells in human samples

Methods of detecting CD34 negative hematopoietic stem cells in a blood sample are described that include (a) removing CD34 positive cells from the blood sample thereby producing a modified blood sample; (b) extracting CD133 positive cells from the modified blood sample thereby producing a fraction c...

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Bibliographische Detailangaben
Hauptverfasser: Dorfner, Mark, Wang, Fu-Sheng, Hirai, Kojiro, Hamaguchi, Yukio
Format: Patent
Sprache:eng
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Zusammenfassung:Methods of detecting CD34 negative hematopoietic stem cells in a blood sample are described that include (a) removing CD34 positive cells from the blood sample thereby producing a modified blood sample; (b) extracting CD133 positive cells from the modified blood sample thereby producing a fraction comprising CD34 negative hematopoietic stem cells; and (c) analyzing the fraction with an automated hematology analyzer, thereby detecting the CD34 negative hematopoietic stem cells.