Method for screening restriction endonucleases

A method is provided for identifying a restriction endonuclease that includes: screening a target DNA sequence for the presence of known methylase sequence motifs, identifying any open reading frames which lie close to the screened methylase sequence motif and assaying the protein products of the op...

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Bibliographische Detailangaben
Hauptverfasser: Roberts, Richard, Byrd, Devon, Morgan, Richard, Patti, Jay, Noren, Christopher
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method is provided for identifying a restriction endonuclease that includes: screening a target DNA sequence for the presence of known methylase sequence motifs, identifying any open reading frames which lie close to the screened methylase sequence motif and assaying the protein products of the open reading frames for restriction endonuclease activity.