Staphylococcus aureus-specific detection media and identifying and/or counting method using same

The present invention relates to a medium for the specific detection of Staphylococcus aureus and/or for the discrimination of coagulase-positive Staphylococcus compared to coagulase-negative Staphylococcus, making it possible to isolate bacteria of the staphylococci genus and to identify the species Staphylococcus aureus , which use at least one enzyme substrate, preferably a chromogenic or fluorescent agent, and even more preferably an indoxyl-based or naphthol-based agent. It also relates to a method for identifying, and optionally counting, Staphylococcus aureus , which uses such a medium. It consists of a Staphylococcus aureus culture medium and at least one enzyme substrate for demonstrating -glucosidase activity. The invention finds a preferred application in the diagnostic field.