Luciferase detection reagent kits and luciferase detection method using the kit

The object of the present invention is to provide a detection method and a method of manufacturing a detection kit which are characterized by use of an organic sulfur reagent, are effective at low concentration of the reagent, are inexpensive and have reduced unpleasant odor. Disclosed is a reagent kit for detecting a Coleoptera luciferase, comprising an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion. A method for detecting a Coleoptera luciferase, comprising step 1 of mixing an aqueous solution containing an organic sulfur reagent having the atomic sequence of sulfur-carbon-sulfur in its chemical structure, a luciferin, adenosine triphosphate and a magnesium ion with a sample containing a Coleoptera luciferase to give a mixed solution and step 2 of measuring the light emitted in the mixed solution is also disclosed.