E2 subunit vaccine comprising recombinant pestivirus E2 protein
The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in g...
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| Format: | Patent |
| Sprache: | eng |
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| Zusammenfassung: | The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×10
5
to 5×10
6
cells/ml with an m.o.i of |
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