Method for the preparation of enantiomerically enriched amines

Method for the preparation of an enantiomerically enriched acylated amine wherein a phenylacetic acid derivative is brought into contact with a mixture of enantiomers of an amine H 2 NCR 1 R 2 R 3 , in which R 1 , R 2 , R 3 are not equal to each other and each independently stand for H, CN, a whether or not substituted (cyclo)alkyl, ary-, alkylaryl or arylalkyl group, whether or not cyclic heteroalkyl or heteroaryl group with one or more N, O or S atoms or in which R 1 and R 2 (and R 3 ) together with the C-atom to which they have been bound form a (bi)cyclic group that optionally contains one or more N, O of S atoms, in the presence of a Pen-G acylase derived from Alcaligenes faecalis. And method for the preparation of enantiomerically enriched amine of formula H 2 NCR 1 R 2 R 3 , in which R 1 , R 2 , R 3 are as defined above, wherein an acylated amine is brought into contact with a Pen-G acylase originating from Alcaligenes faecalis . Preferably enantiomerically enriched phenylglycine amide or an ester of p-hydroxyphenylglycine is applied as phenylacetic acid derivative.