Multilayered microfluidic DNA analysis system and method

A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-she...

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Bibliographische Detailangaben
Hauptverfasser: Briscoe, Cynthia, Yu, Huinan, Grodzinski, Piotr, Marrero, Robert, Burdon, Jeremy, Huang, Rong-Fong
Format: Patent
Sprache:eng
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Zusammenfassung:A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.