Fluorescent nucleotides and labeling methods using the same
Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X-Y-Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represent...
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Zusammenfassung: | Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X-Y-Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R
1
to R
4
represent an alkyl group; V
1
to V
6
represent a hydrogen atom or a substituent; L
1
to L
7
represent a methine group; W represents an oxygen atom, a sulfur atom, -C(R
3
)(R
4
)- or -N(R
5
)- wherein R
3
to R
5
represents an alkyl group; Q represents a nitrogen atom or -C(V
7
)- wherein V
7
represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R
1
or R
2
.
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