METHODS FOR CONTROLLING GIBBERELLIN LEVELS

Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3- hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or control...

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Bibliographische Detailangaben
Hauptverfasser: BROWN, SHERRI M, ELICH, TEDD D, HECK, GREGORY R, KISHORE, GANESH M, LOGUSCH, EUGENE W, LOGUSCH, SHERRY J, PILLER, KENNETH J, RAO, SUDABATHULA, REAM, JOEL E
Format: Patent
Sprache:eng
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Zusammenfassung:Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3- hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3 -hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2 ,3 -hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.