METHOD OF CREATING TRANSFORMED MAIZE

A method of creating a transformed maize plant is disclosed. One embodiment of this method includes the steps of isolating a maize embryo, culturing that embryo to produce callus cells, and bombarding the callus cells with particles coated with copies of a nucleic acid construct. In another embodime...

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Bibliographische Detailangaben
Hauptverfasser: BRAR, GURDIP S, COHEN, BARRY A
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method of creating a transformed maize plant is disclosed. One embodiment of this method includes the steps of isolating a maize embryo, culturing that embryo to produce callus cells, and bombarding the callus cells with particles coated with copies of a nucleic acid construct. In another embodiment of the invention, the cultured embryo produces a mixture of embryogenic and organogenic callus. The nucleic acid-coated particles bombard this mixture, and a transformed plant is produced.