SYNTHETIC GENE FOR EXPRESSING SM-14 IN PICHIA PASTORIS, METHODS FOR PRODUCING AND PURIFYING SM-14 AND THE USE THEREOF AS A VACCINE AND DIAGNOSTIC MEDIUM

Pichia pastorisSchistosoma mansoniPichia pastoris P. pastoris The present invention pertains to the field of production of recombinant proteins, using a synthetic gene for increased expression of the protein in . More specifically, the invention describes the production of a recombinant Sm14 protein of , by providing a synthetic gene for increased production of Sm14 protein, producing said gene and genetically manipulating the strain with said gene in order to produce a vaccine effectively. Improved methods are also provided for producing and purifying this protein from cells, which can be scaled for industrial production.