An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification

[EN] Electrochemical impedance spectroscopy (EIS) has been used for monitoring the enzymatic pineapple waste hydrolysis process. The system employed consists of a device called Advanced Voltammetry, Impedance Spectroscopy & Potentiometry Analyzer (AVISPA) equipped with a specific software application and a stainless steel double needle electrode. EIS measurements were conducted at different saccharification time intervals: 0, 0.75, 1.5, 6, 12 and 24 h. Partial least squares (PLS) were used to model the relationship between the EIS measurements and the sugar determination by HPAEC-PAD. On the other hand, artificial neural networks: (multilayer feed forward architecture with quick propagation training algorithm and logistic-type transfer functions) gave the best results as predictive models for glucose, fructose, sucrose and total sugars. Coefficients of determination (R2 ) and root mean square errors of prediction (RMSEP) were determined as R2 > 0.944 and RMSEP < 1.782 for PLS and R2 > 0.973 and RMSEP < 0.486 for artificial neural networks (ANNs), respectively. Therefore, a combination of both an EIS-based technique and ANN models is suggested as a promising alternative to the traditional laboratory techniques for monitoring the pineapple waste saccharification step Financial support from the European FEDER and the Spanish government (MAT2012-34829-C04-04), the Generalitat Valenciana (PROMETEOII/2014/047) and the FPI-UPV Program funds are gratefully acknowledged. Conesa Domínguez, C.; Ibáñez Civera, FJ.; Seguí Gil, L.; Fito Maupoey, P.; Laguarda-Miro, N. (2016). An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification. Sensors. 16(188). https://doi.org/10.3390/s16020188