Complete sequence of three different biotypes of Tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain

[EN] Tomato spotted wilt virus (TSWV) is worldwide spread and causes production losses in many important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the most successful method for disease control. So far, only two resistance genes have been found to confer resistance against a wide spectrum of TSWV isolates: Sw-5 in tomato and Tsw in pepper. However, TSWV resistance-breaking isolates have emerged in different countries after a few years of implementation of resistant cultivars. In this paper we report the first complete nucleotide sequence of three TSWV isolates from Spain with different biotypes according to the ability of overcoming resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 resistance breaking, SBR) and PVR (Tsw resistance-breaking, TBR). The genome of these TSWV isolates consisted of three genomic segments: L (8913-8914 nt), M (4752-4825 nt) and S (2924-2961 nt). Variations in nucleotide sequences and genomic lengths among the different virus biotypes are reported here. Phylogenetic analysis of the five TSWV open reading frames showed evidences of reassortment between genomic segments of LL-N.05 and Pujol1TL3, which was supported by analysis with different recombination-detecting algorithms. Genetic distances were uncorrelated to biotype, host or geographic location. D.E.D. was the recipient of a predoctoral FPU fellowship from the Spanish Ministry of Education, Culture and Sports. This work was funded in part by grants RTA2008-00010-C03 and RTA2013-00047-C02 from Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) and grants ACOMP/2009/103, ACOMP/2010/085 and ACOMP/2011/078 from Generalitat Valenciana. Work in the J.A.D. laboratory was supported by grant BIO2011-26741 from the Spanish Ministerio de Economia y Competitividad. We thank Veronica Aragones (IBMCP, CSIC-UPV) for technical assistance. Debreczeni, DE.; López Del Rincón, C.; Aramburu, J.; Daros Arnau, JA.; Soler Aleixandre, S.; Galipienso Torregrosa, L.; Falk, BW... (2015). Complete sequence of three different biotypes of Tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Archives of Virology. 160:2117-2123. https://doi.org/10.1007/s00705-015-2453-8 de Haan P, Kormelink R, de Oliveira Resende R, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. J Gen Virol 72:2207–2216 de Haan P, Wagemakers L