Citrus psorosis virus 24K protein inhibits the processing of miRNA precursors by interacting with components of the biogenesis machinery

[EN] Sweet orange (Citrus sinensis) is one of the most important fruit crops worldwide. Virus infections in this crop can interfere with cellular processes, causing dramatic economic losses. By performing RT-qPCR analyses, we demonstrated that citrus psorosis virus (CPsV)-infected orange plants exhi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Marmisolle, Facundo E, Borniego, Maria B, Cambiagno, Damián A, Gonzalo, Lucía, García, Maria L, Manavella, Pablo A, Hernández Fort, Carmen, Reyes, Carina A
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:[EN] Sweet orange (Citrus sinensis) is one of the most important fruit crops worldwide. Virus infections in this crop can interfere with cellular processes, causing dramatic economic losses. By performing RT-qPCR analyses, we demonstrated that citrus psorosis virus (CPsV)-infected orange plants exhibited higher levels of unprocessed microRNA (miRNA) precursors than healthy plants. This result correlated with the reported reduction of mature miRNAs species. The protein 24K, the CPsV suppressor of RNA silencing (VSR), interacts with miRNA precursors in vivo. Thus, this protein becomes a candidate responsible for the increased accumulation of unprocessed miRNAs. We analyzed 24K RNA-binding and protein-protein interaction domains and described patterns of its subcellular localization. We also showed that 24K colocalizes within nuclear D-bodies with the miRNA biogenesis proteins DICER-LIKE 1 (DCL1), HYPONASTIC LEAVES 1 (HYL1), and SERRATE (SE). According to the results of bimolecular fluorescence complementation and co-immunoprecipitation assays, the 24K protein interacts with HYL1 and SE. Thus, 24K may inhibit miRNA processing in CPsV-infected citrus plants by direct interaction with the miRNA processing complex. This work contributes to the understanding of how a virus can alter the regulatory mechanisms of the host, particularly miRNA biogenesis and function. Agencia Nacional de Promocion de la Investigacion, el Desarrollo Tecnologico y la Innovacion (Agencia I+D+i) PICT 2017-1233 Carina A. Reyes. Agencia Nacional de Promocion de la Investigacion, el Desarrollo Tecnologico y la Innovacion (Agencia I+D+i) PICT 2018-1264 Maria L. Garcia. Agencia Nacional de Promocion de la Investigacion, el Desarrollo Tecnologico y la Innovacion (Agencia I+D+i) PICT 2020-01925 Carina A. Reyes. Proyecto de Cooperacion Cientifica para el Desarrollo (Ministerio de Economia, Industria y Competitividad) COOPA20191 Carina A. Reyes y Carmen Hernandez. Marmisolle, FE.; Borniego, MB.; Cambiagno, DA.; Gonzalo, L.; García, ML.; Manavella, PA.; Hernández Fort, C... (2024). Citrus psorosis virus 24K protein inhibits the processing of miRNA precursors by interacting with components of the biogenesis machinery. Microbiology Spectrum. 12(7). https://doi.org/10.1128/spectrum.03513-23