Implementación de un set de marcadores microsatélites para la diferenciación de variedades de Fragaria x ananassa

[EN] Cultivated strawberry (Fragaria x ananassa) is a high economic value horticultural crop from which there are more than one thousand varieties around the world. To maintain the relevance of the crop, to avoid the dependence on foreign material and to be adapted to the market and customer needs, new strawberry varieties are being developed through breeding programs. With the aim of defending breeder s rights in the obtained varieties and to differentiate appropriately the genotypes among them, molecular markers have been generated to evaluate accurately the genetic content of each variety. In this project, a set of microsatellite molecular markers has been selected and used to carry out the varietal differentiation of cultivated strawberry and the efficacy of the markers has been compared with the results obtained for the same varieties with RAPDs and ISSR markers previously used. The transferability of microsatellites in raspberry (Rubus idaeus) has been studied since it belongs to the same subfamily as cultivated strawberry. Furthermore, the association of microsatellites with agronomical traits of commercial interest such as runner production, fruit firmness and short-day have been tested. Finally, it has been done a bibliographic revision about possible future projects to strengthen the development of genetics studies in Valgenetics S.L. [ES] La fresa cultivada (Fragaria x ananassa) es una fruta de alto valor económico de la que existen más de mil variedades en todo el mundo. Para mantener la importancia del cultivo, evitar la dependencia de material foráneo y adaptarse a las necesidades del mercado y del consumidor, se están obteniendo nuevas variedades de fresa mediante programas de mejora. Con el fin de defender los derechos del obtentor sobre las variedades generadas y poder diferenciar debidamente unos genotipos de otros, se han desarrollado marcadores moleculares que permiten evaluar adecuadamente el contenido genético de cada variedad. En el presente trabajo se han seleccionado y utilizado un set de marcadores moleculares microsatélites para realizar la diferenciación de variedades de fresa y se ha comparado su eficacia con los resultados obtenidos para las mismas variedades con los marcadores RAPD e ISSR. Se ha estudiado la transferibilidad del mismo set de microsatélites en frambuesa (Rubus idaeus), ya que es de la misma subfamilia que la fresa cultivada. Además, se ha evaluado la asociación de los microsatélites a caracteres agronómicos de