The investigation of the mutagenic activity of Kars River sediments on Orthrias angorae (Steindachner, 1897)

Sediments collected in Kars River were tested for mutagenicity by means of peripheral erythrocyte micronucleus frequency in Orthrias angorae. Micronuclei frequencies (MN) of all the groups exposed to the sediments were higher than those of the control group. Statistical analysis showed significant d...

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Veröffentlicht in:Veteriner fakultesi dergisi 2009, Vol.15 (1), p.35-40
Hauptverfasser: Özkan, O., Kafkas Univ., Faculty of Veterinary Medicine, Kars (Turkey). Div. of Pharmacology and Toxicology, Gül, S., Kafkas Univ., Faculty of Science and Letters, Kars (Turkey). Div. of Biology, Keleş, O., İstanbul Univ., Faculty of Veterinary Medicine, İstanbul (Turkey). Div. of Pharmacology, Aksu, P., Kafkas Univ., Faculty of Veterinary Medicine, Kars (Turkey). Div. of Pharmacology and Toxicology, Kaya, T.Ö., Kafkas Univ., Faculty of Veterinary Medicine, Kars (Turkey). Div. of Histology and Embryology, Nur, G., Kafkas Univ., Faculty of Veterinary Medicine, Kars (Turkey). Div. of Histology and Embryology
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Zusammenfassung:Sediments collected in Kars River were tested for mutagenicity by means of peripheral erythrocyte micronucleus frequency in Orthrias angorae. Micronuclei frequencies (MN) of all the groups exposed to the sediments were higher than those of the control group. Statistical analysis showed significant differences between the control and the treatment groups (P 0.001). The MN frequencies of the blood samples in three regions (Selim, Pasacayir, Bogazkoy) on the day 6 have a trend to increase against to control. MN frequencies of samples collected from 36th hours sediment exposure increased in the three districts (Selim, Pasacayir, Bogazkoy) when compared to control groups. On the other hand there is a decrease in only one region (Kars) at 6 days. This study evaluates for the first time the mutagenic load of sediments collected along the Kars River and provides evidence that the presence of genotoxic agents in river sediments correlates with the genotoxic damage (micronucleated erythrocytes) in fish collected from Kars River. Sediment numuneleri, Orthrias angorae'da peripheral eritrosit mikronukleus testi yoluyla mutajenite testi için Kars çayından elde edildi. Mikronuklei frekansı (MN), kontrol grubuna göre sedimente maruz kalan tüm gruplarda daha yüksek bulundu. Kontrol ve deneme grupları arasındaki fark istatiksel olarak önemli görülmüştür (P 0.001). Üç bölgeden (Selim, Paşaçayır, Boğazköy), sedimente maruz kalan gruplardan 6. günde alınan kan numunelerinin MN frekansları kontrol grubuna göre yüksek bulunmuştur. Sedimente maruz kalan üç bölgeye ait 36. saat numuneleri MN frekansları control grubu ile kıyaslandığında yüksek bulunmuştur. Diğer yandan 6. günde tek bir numune bölgesinde (Kars) bir azalma görülmüştür. Bu çalışma ile Kars çayından elde edilen sedimentin mutajenik yükü ve balıklarda genotoksik hasar (mikronukleili eritrositler) ile sediment içerisindeki genotoksik ajanların varlığına dair kanıtlar ilk kez değerlendirilmiştir.
ISSN:1309-2251
1300-6045
1309-2251