Investigation of chicken infectious anemia virus infection by PCR and ELISA in chicken flocks

Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIA...

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Veröffentlicht in:	Turkish journal of veterinary & animal sciences 2008-01, Vol.32 (2), p.79-84
Hauptverfasser:	Hadimli, H.H., Selçuk Univ., Faculty of Veterinary Medicine, Konya (Turkey). Div. of Microbiology, Erganiş, O., Selçuk Univ., Faculty of Veterinary Medicine, Konya (Turkey). Div. of Microbiology, Güler, L., Veterinary Control and Research Institute, Konya (Turkey), Uçan, U.S., Selçuk Univ., Faculty of Veterinary Medicine, Konya (Turkey). Div. of Microbiology
Format:	Artikel
Sprache:	eng
Schlagworte:	anaemia antibodies antibody testing antikor antikor testleri bağışıksal tanı broilers CHICKENS CIRCOVIRIDAE diagnosis Diagnosis of Animal Diseases disease prevalence disease surveys ELISA epidemiological surveys epidemiology epidemiyoloji epidemiyolojik araştırmalar Et Veren Hayvanlar etlik piliç hastalık tetkiki hastalık yaygınlığı Hayvan Hastalıkları Teşhisi Hayvanların Prion, Viral, Bakteriyel ve Fungal Patojenleri http://www.fao.org/aos/agrovoc#c_1540 http://www.fao.org/aos/agrovoc#c_15968 http://www.fao.org/aos/agrovoc#c_23352 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_3791 http://www.fao.org/aos/agrovoc#c_8013 IDENTIFICACION IDENTIFICATION immunodiagnosis kansızlık kümes hayvanları Meat Producing Animals PCR polimeraz zincir reaksiyonu POLLO polymerase chain reaction POULET poultry Prion, Viral, Bacterial and Fungal Pathogens of Animals serological surveys serolojik etüdler seroprevalans seroprevalence Techniques and Methodology Teknik ve Metodoloji TEST ELISA teşhis TURKEY TURQUIA TURQUIE Türkiye
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Zusammenfassung:	Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples from 20 flocks (80.0%) CIAV-specific DNA was detected. In commercial layers antibodies were detected in 15 (93.7%) of 16 flocks and viral DNA was detected in 13 (86.6%) of 15 flocks. In commercial broilers both CIAV antibodies and DNA were detected in 7 (70%) of 10 şocks tested. While seropositivity was detected in 12 (100%) of breeding broilers (8) and layer (4) flocks tested, no PCR was performed in these flocks. The study showed high prevalence of subclinical CIAV infection in the investigated chicken flocks. Bu çalışmada, Türkiye'nin bazı illerinde tavukçuluk işletmelerinde chicken infectious anemia virus (CIAV) enfeksiyonu ELISA ve PCR ile araştırıldı. Toplam 38 kümesten (16 ticari yumurtacı, 10 ticari broyler, 4 damızlık yumurtacı ve 8 damızlık broyler) 922 serum örneği, ticari bir kompetetiv ELISA kiti kullanarak CIAV antikorları yönünden test edildi. Otuz dört işletmeden 609 (% 66,0) serum örneğinde CIAV antikorları pozitif bulundu. Yirmi beş işletmeden 95 timus örneği (15 ticari yumurtacı işletmesinden 57 örnek ve 10 ticari broyler işletmesinden 38 örnek) PCR ile test edildi. Yirmi işletmeden (% 80,0) 53 (% 55,8) timus örneğinde CIAVspesifik DNA tespit edildi. Ticari yumurtacılarda, 16 işletmeden 15'inde (% 93,7) antikorlar ve 15 işletmeden 13'ünde (% 86,6) DNA belirlendi. Ticari broylerlerde, test edilen 10 işletmeden 7'sinde (% 70,0) hem CIAV antikorları hemde DNA tespit edildi. On iki broyler (8) ve yumurtacı (4) damızlık kümeslerin hepsinde (% 100) sero-pozitiflik belirlenirken, bu kümeslerde PCR yapılmadı. Bu çalışma, araştırılan tavuk işletmelerinde CIAV enfeksiyonunun subklinik olarak yüksek prevalansta olduğunu gösterdi.
ISSN:	1303-6181 1300-0128