Presence of a very virulent genotype of Infectious Bursal Disease Virus in vaccinated layer hens in Turkey

Despite vaccinations against very virulent (vv) strains of infectious bursal disease virus (IBDV) or Gumboro, Gumboro outbreaks in vaccinated hens in Turkey are still reported. These outbreaks suggested that new vv strains might have emerged and the present vaccines might not be able to provide prot...

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Veröffentlicht in:Turkish journal of veterinary & animal sciences 2007-01, Vol.31 (2), p.105-111
Hauptverfasser: Çeribaşı, A.O., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Pathology, Bulut, H., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Virology, Gülaçtı, İ., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Virology, Eröksüz, Y., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Pathology, Bolat, Y., Fırat University, Faculty of Veterinary Medicine, Elazığ (Turkey). Div. of Virology
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Zusammenfassung:Despite vaccinations against very virulent (vv) strains of infectious bursal disease virus (IBDV) or Gumboro, Gumboro outbreaks in vaccinated hens in Turkey are still reported. These outbreaks suggested that new vv strains might have emerged and the present vaccines might not be able to provide protection against infections with these strains. The objective of this study was to characterize the viruses isolated from vaccinated layer hens. For this purpose, the hypervariable region of the VP2 gene of the viruses was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and the amplification products were sequenced. These results showed that the field isolates of IBDV were more closely related to the European and Asian vv strain rather than the classical virulent strain, and the Gumboro outbreaks in Turkey were not derived from a new vv strain. Türkiye'de enfeksiyöz bursal hastalık virusu (IBDV) yada Gumboro'nun oldukça virulent (vv) suşlarına karşı aşılamalara rağmen, aşılanmış tavuklarda IBDV salgınları hala rapor edilmektedir. Bu salgınlar, mevcut aşıların korumadığı, yeni vv türlerin ortaya çıkmış olabileceğini akla getirmektedir. Bu çalışmada, aşılı yumurtacı tavuklardan izole edilen virusların karakterizasyonu amaçlanmıştır. Bu amaçla, VP2 gen bölgesinin oldukça değişken bölgesi tersine transkripsiyon-polimeraz zincir tepkimesi (RT-PCR) ile çoğaltıldı ve çoğaltılan ürünler sekanslandı. Bu sonuçlara göre, IBDV'nin saha izolatlarının klasik virulent suşdan ziyade Avrupa ve Asya vv suşlarına daha yakın ilişkili olduğu ve salgınların yeni bir vv suşundan köken almadığı görülmüştür.
ISSN:1303-6181
1300-0128