Embryo culture in Barley (Hordeum vulgare L.)

The effects of medium and sterilization on the mature embryo culture of barley (Hordeum vulgare L.) were studied. Among the different media used, the highest plantlet development from embryos was obtained in Randolph and Cox medium (92.1%), followed by Murashige and Skoog (MS) (91.3%), half-strength...

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Veröffentlicht in:	Turkish journal of biology 2002, Vol.26 (3), p.175-180
Hauptverfasser:	POYRAZOĞLU, Esin, Çoban, BÜRÜN, Betül
Format:	Artikel
Sprache:	eng
Schlagworte:	antibiotics antibiyotikler arpa barley Bitki Materyallerinin İn Vitro Kültürü cıva klorür comparisons culture media embriyo kültürü embryo culture Hordeum vulgare in vitro culture in vitro Culture of Plant Material in vitro kültür karşılaştırmalar kültür ortamı mercurous chloride sodium hypochlorite sodyum hipoklorit sterilize etme sterilizing Techniques and Methodology Teknik ve Metodoloji
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Zusammenfassung:	The effects of medium and sterilization on the mature embryo culture of barley (Hordeum vulgare L.) were studied. Among the different media used, the highest plantlet development from embryos was obtained in Randolph and Cox medium (92.1%), followed by Murashige and Skoog (MS) (91.3%), half-strength MS (1/2 MS) (87.7%) and B5 (85.7%). Among the sterilization methods studied, sodium hypochlorite sterilization followed by treatment of antibiotic solution and sterilization HgC$l_2$ application was found to be more effective than the other methods. However, no statistically significant negative effect of HgC$l_2$ on the plantlet development from embryos was observed.
ISSN:	1300-0152 1303-6092