Histological and immunohistochemical studies on the Furstenberg's Rosette in cows

The purpose of this study was to investigate the light and electron microscopic structure of Furstenberg's rosette region and to determine of uptake of macromolecules from follicle associated epithelium (FAE) of healthy cows in lactation period. In this study, 26 distal teat end taken from 10 animals in lactation period were used as material. Tissue samples were prepared for examination by light and transmission electron microscope. Indirect immunoperoxidase method was implemented to determine of cells containing IgA and IgG. Ferritin solution (25 mg/ml) was applied to distal teat end 1 h before slaughtered to show uptake of macromolecules in FAE. It was observed that Furstenberg's rosette region consists of lymphoid and nonlymphoid areas. Solitary and aggregate lymphoid follicles were seen in lymphoid areas. It was attracted attention that upper surfaces of these follicles were covered with FAE. IgA positive cells were seen in centrum germinativum of lymphoid follicles in the lymphoid region and subepithelial areas. High endothelial venules (HEV), which is characteristic feature of lympho-epithelial tissues, were observed at connective tissue which is around of the lymphoid follicles. In thin sections, the teat end tissue have been previously applied to ferritin, ferritin particles were detected in apical invaginations and pinocytotic vesicles of membranous cells (M cells). It was determined that there is Furstenbeg's rosette associated lymphoid tissue (FALT) in teat end and this region was generated spesific immune response against antigens after their uptaking and proccessing. Thus, we have reached conclusion that FALT may play a role mucosal immunity of teat end. Araştırma laktasyon dönemindeki sağlıklı ineklerde Furstenberg rozeti bölgesinin yapısını ışık ve elektron mikroskobik düzeylerde incelemek ve folikül ile ilişkili epitel (FAE)'den makromoleküllerin alınışını belirlemek amacıyla yapıldı. Çalışmada materyal olarak 10 adet sağlıklı hayvandan alınan 26 meme başı kullanıldı. Doku örnekleri ışık ve elektron mikroskobik incelemeler için hazırlandı. IgA ve IgG içeren hücrelerin belirlenmesi için indirekt immunperoksidaz metodu uygulandı. FAE tarafından makromoleküllerin alınışını göstermek için, kesimden 1 saat önce distal meme ucuna ferritin solüsyonu (25 mg/ml) uygulandı. Furstenberg rozetinin lenfoid ve nonlenfoid bölgelerden oluştuğu gözlendi. Lenfoid bölgede soliter ve agregat lenf folikülleri görüldü. Bu foliküllerin üstünün FAE ile örtülü oldu