Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin β1 and γ1 chains

In the present study, the chain specificity of 16 commonly used monoclonal antibodies to human laminin(s) was analysed by using recombinant laminin β1 and γ1 chains. By ELISA, all antibodies reacted with purified placenta laminin, and most antibodies recognised either recombinant β1 or γ1 chains. Reactivity and chain specificity was confirmed against the recombinant chains in Western blotting under non-reducing conditions, and only a few antibodies were reactive under reducing conditions. Most antibodies were able to immunoprecipitate associated laminin β1/γ1 chains from platelet lysates. Based on these results and data from the literature, a tentative epitope map is presented.