Structure of the O-polysaccharide from the lipopolysaccharide from Vibrio cholerae O6

Structure of the O-polysaccharide from the lipopolysaccharide from Vibrio cholerae O6

The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof....

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Veröffentlicht in:Carbohydrate research 2002-04, Vol.337 (9), p.813-817
Hauptverfasser: Bergström, Niklas, Nair, G.Balakrish, Weintraub, Andrej, Jansson, Per-Erik
Format: Artikel
Sprache:eng
Schlagworte:
Bacterial polysaccharide
Carbohydrate Sequence
Hydrolysis
Lipopolysaccharide
Lipopolysaccharides - chemistry
Lipopolysaccharides - metabolism
Magnetic Resonance Spectroscopy
Medicin och hälsovetenskap
Methylation
Molecular Sequence Data
O Antigens - analysis
O Antigens - chemistry
O Antigens - metabolism
O-Antigen
Vibrio cholerae - chemistry
Vibrio cholerae diarrhea
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Zusammenfassung:The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof. The O-polysaccharide had the following tetrasaccharide repeating unit. Two O-acetyl groups are present, one of them making the GlcNAc residue fully substituted and the steric crowding considerable at the branching residue. Graphic
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(02)00056-3