Evaluation of the BacT/ALERT 3D system for recovery and drug susceptibility testing of Mycobacterium tuberculosis

We evaluated the BacT/ALERT 3D system for recovery and drug susceptibility testing (DST) of Mycobacterium tuberculosis (MTB). Of 2659 clinical specimens, MTB was detected in 92 using BacT/ALERT, compared to 94 using Löwenstein–Jensen culture. Detection time was 25% shorter with BacT/ALERT. Sensitivi...

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Veröffentlicht in:	Clinical microbiology and infection 2003-11, Vol.9 (11), p.1148-1152
Hauptverfasser:	Ängeby, K.A.K., Werngren, J., Toro, J.C., Hedström, G., Petrini, B., Hoffner, S.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial diseases Biological and medical sciences diagnosis drug susceptibility testing ethambutol Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Infectious diseases isoniazid Medical sciences Medicin och hälsovetenskap Microbial Sensitivity Tests - methods Microbial Sensitivity Tests - standards Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - isolation & purification new methods Reagent Kits, Diagnostic - standards rifampicin Sensitivity and Specificity Specimen Handling - methods streptomycin Tuberculosis Tuberculosis - microbiology Tuberculosis and atypical mycobacterial infections
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Zusammenfassung:	We evaluated the BacT/ALERT 3D system for recovery and drug susceptibility testing (DST) of Mycobacterium tuberculosis (MTB). Of 2659 clinical specimens, MTB was detected in 92 using BacT/ALERT, compared to 94 using Löwenstein–Jensen culture. Detection time was 25% shorter with BacT/ALERT. Sensitivities were 92%, 96%, 78% and 100% for resistance to rifampicin, isoniazid, streptomycin and ethambutol, respectively, while specificity was 100% for all antibiotics, when BacT/ALERT was compared with the BACTEC 460 method on 50 MTB isolates. The BacT/ALERT system is fully automated and creates no radioactive waste. It seems to be a valid alternative for primary isolation, but further evaluation is needed regarding DST.
ISSN:	1198-743X 1469-0691
DOI:	10.1046/j.1469-0691.2003.00783.x