Natural killer cell education in mice with single or multiple major histocompatibility complex class I molecules

The ability of murine NK cells to reject cells lacking self MHC class I expression results from an in vivo education process. To study the impact of individual MHC class I alleles on this process, we generated mice expressing single MHC class I alleles (K(b), D(b), D(d), or L(d)) or combinations of...

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Veröffentlicht in:The Journal of experimental medicine 2005-04, Vol.201 (7), p.1145-1155
Hauptverfasser: Johansson, Sofia, Johansson, Maria, Rosmaraki, Eleftheria, Vahlne, Gustaf, Mehr, Ramit, Salmon-Divon, Mali, Lemonnier, François, Kärre, Klas, Höglund, Petter
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container_issue 7
container_start_page 1145
container_title The Journal of experimental medicine
container_volume 201
creator Johansson, Sofia
Johansson, Maria
Rosmaraki, Eleftheria
Vahlne, Gustaf
Mehr, Ramit
Salmon-Divon, Mali
Lemonnier, François
Kärre, Klas
Höglund, Petter
description The ability of murine NK cells to reject cells lacking self MHC class I expression results from an in vivo education process. To study the impact of individual MHC class I alleles on this process, we generated mice expressing single MHC class I alleles (K(b), D(b), D(d), or L(d)) or combinations of two or more alleles. All single MHC class I mice rejected MHC class I-deficient cells in an NK cell-dependent way. Expression of K(b) or D(d) conveyed strong rejection of MHC class I-deficient cells, whereas the expression of D(b) or L(d) resulted in weaker responses. The educating impact of weak ligands (D(b) and L(d)) was further attenuated by the introduction of additional MHC class I alleles, whereas strong ligands (K(b) and D(d)) maintained their educating impact under such conditions. An analysis of activating and inhibitory receptors in single MHC class I mice suggested that the educating impact of a given MHC class I molecule was controlled both by the number of NK cells affected and by the strength of each MHC class I-Ly49 receptor interaction, indicating that NK cell education may be regulated by a combination of qualitative and quantitative events.
doi_str_mv 10.1084/jem.20050167
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subjects Alleles
Animals
Antigens, Ly - metabolism
Down-Regulation
Flow Cytometry
Fluoresceins
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class I - metabolism
Killer Cells, Natural - metabolism
Lectins, C-Type
Mice
Mice, Transgenic
Receptors, Immunologic - metabolism
Receptors, Natural Killer Cell
Receptors, NK Cell Lectin-Like
Self Tolerance - genetics
Self Tolerance - immunology
Succinimides
title Natural killer cell education in mice with single or multiple major histocompatibility complex class I molecules
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