RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing

Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed ch...

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Veröffentlicht in:Genes & development 2005-10, Vol.19 (19), p.2301-2306
Hauptverfasser: Djupedal, Ingela, Portoso, Manuela, Spåhr, Henrik, Bonilla, Carolina, Gustafsson, Claes M, Allshire, Robin C, Ekwall, Karl
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Sprache:eng
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Zusammenfassung:Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in pre-siRNA transcription. We define a centromeric pre-siRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing.
ISSN:0890-9369
1549-5477
1549-5477
DOI:10.1101/gad.344205