Genetic and biochemical studies of SNPs of the mitochondrial Aβ-degrading protease, hPreP
Several studies suggest mitochondrial dysfunction as a possible mechanism underlying the development of Alzheimer disease (AD). There is data showing that amyloid-β (Aβ) peptide is present in AD brain mitochondria. The human presequence protease (hPreP) was recently shown to be the major mitochondri...
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Veröffentlicht in: | Neuroscience letters 2010, Vol.469 (2), p.204-208 |
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Sprache: | eng |
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Zusammenfassung: | Several studies suggest mitochondrial dysfunction as a possible mechanism underlying the development of Alzheimer disease (AD). There is data showing that amyloid-β (Aβ) peptide is present in AD brain mitochondria. The human presequence protease (hPreP) was recently shown to be the major mitochondrial Aβ-degrading enzyme. We investigated if there is an increased susceptibility to AD, which can be attributed to genetic variation in the hPreP gene
PITRM1 and if the proteolytic efficiency of recombinant hPreP variants is affected. When a total of 673 AD cases and 649 controls were genotyped for 18 single nucleotide polymorphisms (SNPs), no genetic association between any of the SNPs and the risk for AD was found. In contrast, functional analysis of four non-synonymous SNPs in hPreP revealed a decreased activity compared to wild type hPreP. Using Aβ, the presequence of ATP synthase F
1β subunit and a fluorescent peptide as substrates, the lowest activity was observed for the hPreP(A525D) variant, corresponding to rs1224893, which displayed only 20–30% of wild type activity. Furthermore, the activity of all variants was restored by the addition of Mg
2+, suggesting an important role for this metal during proteolysis. In conclusion, our data suggest that genetic variation in the hPreP gene
PITRM1 may potentially contribute to mitochondrial dysfunctions. |
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ISSN: | 0304-3940 1872-7972 1872-7972 |
DOI: | 10.1016/j.neulet.2009.11.075 |