Sequence Scrambling in Shotgun Proteomics is Negligible
Analysis of 15,897 low-energy (CAD) and 10,878 higher-energy (HCD) collisional dissociation mass spectra of doubly protonated tryptic peptides taken with high resolution revealed that the rate of sequence scrambling due to b-ion cyclization is negligible (
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Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2011-07, Vol.22 (7), p.1121-1124 |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Analysis of 15,897 low-energy (CAD) and 10,878 higher-energy (HCD) collisional dissociation mass spectra of doubly protonated tryptic peptides taken with high resolution revealed that the rate of sequence scrambling due to b-ion cyclization is negligible ( |
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ISSN: | 1044-0305 1879-1123 1879-1123 |
DOI: | 10.1007/s13361-011-0130-z |