Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles
Abstract Several clinical observations have demonstrated a link between heart rate and anxiety or panic disorders. In these patients, β-adrenergic receptor function was altered. This prompted us to investigate whether the β-adrenergic receptor agonist isoproterenol, at a dose that stimulates periphe...
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| Veröffentlicht in: | Physiology & behavior 2015-04, Vol.142, p.79-84 |
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| creator | Leo, Giuseppina Guescini, Michele Genedani, Susanna Stocchi, Vilberto Carone, Chiara Filaferro, Monica Sisti, Davide Marcoli, Manuela Maura, Guido Cortelli, Pietro Guidolin, Diego Fuxe, Kjell Agnati, Luigi Francesco |
| description | Abstract Several clinical observations have demonstrated a link between heart rate and anxiety or panic disorders. In these patients, β-adrenergic receptor function was altered. This prompted us to investigate whether the β-adrenergic receptor agonist isoproterenol, at a dose that stimulates peripheral β-adrenergic system but has no effects at the central nervous system, can induce anxiety-like behavior in rats. Moreover, some possible messengers involved in the peripheral to brain communication were investigated. Our results showed that isoproterenol (5 mg kg − 1 i.p.) increased heart rate, evoked anxiety-like behavior, did not result in motor impairments and increased extracellular vesicle content in the blood. Plasma corticosterone level was unmodified as well as vesicular Hsp70 content. Vesicular miR-208 was also unmodified indicating a source of increased extracellular vesicles different from cardiomyocytes. We can hypothesize that peripheral extracellular vesicles might contribute to the β-adrenergic receptor-evoked anxiety-like behavior, acting as peripheral signals in modulating the mental state. |
| doi_str_mv | 10.1016/j.physbeh.2015.02.002 |
| format | Article |
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In these patients, β-adrenergic receptor function was altered. This prompted us to investigate whether the β-adrenergic receptor agonist isoproterenol, at a dose that stimulates peripheral β-adrenergic system but has no effects at the central nervous system, can induce anxiety-like behavior in rats. Moreover, some possible messengers involved in the peripheral to brain communication were investigated. Our results showed that isoproterenol (5 mg kg − 1 i.p.) increased heart rate, evoked anxiety-like behavior, did not result in motor impairments and increased extracellular vesicle content in the blood. Plasma corticosterone level was unmodified as well as vesicular Hsp70 content. Vesicular miR-208 was also unmodified indicating a source of increased extracellular vesicles different from cardiomyocytes. We can hypothesize that peripheral extracellular vesicles might contribute to the β-adrenergic receptor-evoked anxiety-like behavior, acting as peripheral signals in modulating the mental state.</description><identifier>ISSN: 0031-9384</identifier><identifier>ISSN: 1873-507X</identifier><identifier>EISSN: 1873-507X</identifier><identifier>DOI: 10.1016/j.physbeh.2015.02.002</identifier><identifier>PMID: 25654993</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Anxiety - blood ; Anxiety - chemically induced ; Anxiety-like behavior ; Blood Chemical Analysis ; Blotting, Western ; Cardiovascular Agents - pharmacology ; Corticosterone - blood ; Electrocardiography ; Extracellular vesicles ; Extracellular Vesicles - drug effects ; Extracellular Vesicles - metabolism ; Heart Rate - drug effects ; Hsp70 ; HSP70 Heat-Shock Proteins - metabolism ; Isoproterenol ; Isoproterenol - pharmacology ; Male ; Medicin och hälsovetenskap ; MicroRNAs - metabolism ; miR-208 ; Motor Activity - drug effects ; Psychiatry ; Psychotropic Drugs - pharmacology ; Rats, Sprague-Dawley</subject><ispartof>Physiology & behavior, 2015-04, Vol.142, p.79-84</ispartof><rights>Elsevier Inc.</rights><rights>2015 Elsevier Inc.</rights><rights>Copyright © 2015 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-48733e1e6105484320419db59d39ae0ad72629850453133e55fc33367376c68a3</citedby><cites>FETCH-LOGICAL-c508t-48733e1e6105484320419db59d39ae0ad72629850453133e55fc33367376c68a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.physbeh.2015.02.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25654993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:130995056$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Leo, Giuseppina</creatorcontrib><creatorcontrib>Guescini, Michele</creatorcontrib><creatorcontrib>Genedani, Susanna</creatorcontrib><creatorcontrib>Stocchi, Vilberto</creatorcontrib><creatorcontrib>Carone, Chiara</creatorcontrib><creatorcontrib>Filaferro, Monica</creatorcontrib><creatorcontrib>Sisti, Davide</creatorcontrib><creatorcontrib>Marcoli, Manuela</creatorcontrib><creatorcontrib>Maura, Guido</creatorcontrib><creatorcontrib>Cortelli, Pietro</creatorcontrib><creatorcontrib>Guidolin, Diego</creatorcontrib><creatorcontrib>Fuxe, Kjell</creatorcontrib><creatorcontrib>Agnati, Luigi Francesco</creatorcontrib><title>Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles</title><title>Physiology & behavior</title><addtitle>Physiol Behav</addtitle><description>Abstract Several clinical observations have demonstrated a link between heart rate and anxiety or panic disorders. In these patients, β-adrenergic receptor function was altered. This prompted us to investigate whether the β-adrenergic receptor agonist isoproterenol, at a dose that stimulates peripheral β-adrenergic system but has no effects at the central nervous system, can induce anxiety-like behavior in rats. Moreover, some possible messengers involved in the peripheral to brain communication were investigated. Our results showed that isoproterenol (5 mg kg − 1 i.p.) increased heart rate, evoked anxiety-like behavior, did not result in motor impairments and increased extracellular vesicle content in the blood. Plasma corticosterone level was unmodified as well as vesicular Hsp70 content. Vesicular miR-208 was also unmodified indicating a source of increased extracellular vesicles different from cardiomyocytes. We can hypothesize that peripheral extracellular vesicles might contribute to the β-adrenergic receptor-evoked anxiety-like behavior, acting as peripheral signals in modulating the mental state.</description><subject>Animals</subject><subject>Anxiety - blood</subject><subject>Anxiety - chemically induced</subject><subject>Anxiety-like behavior</subject><subject>Blood Chemical Analysis</subject><subject>Blotting, Western</subject><subject>Cardiovascular Agents - pharmacology</subject><subject>Corticosterone - blood</subject><subject>Electrocardiography</subject><subject>Extracellular vesicles</subject><subject>Extracellular Vesicles - drug effects</subject><subject>Extracellular Vesicles - metabolism</subject><subject>Heart Rate - drug effects</subject><subject>Hsp70</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Isoproterenol</subject><subject>Isoproterenol - pharmacology</subject><subject>Male</subject><subject>Medicin och hälsovetenskap</subject><subject>MicroRNAs - metabolism</subject><subject>miR-208</subject><subject>Motor Activity - drug effects</subject><subject>Psychiatry</subject><subject>Psychotropic Drugs - pharmacology</subject><subject>Rats, Sprague-Dawley</subject><issn>0031-9384</issn><issn>1873-507X</issn><issn>1873-507X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk-PFCEQxYnRuOPqR9D00Uu30HTRcNFsNv5LNvGgJt4IQ9dkmWGaFuhx59tLZ2bXxMTIBUL9Hi_UK0JeMtowysSbbTPdHtMab5uWMmho21DaPiIrJnteA-1_PCYrSjmrFZfdBXmW0paWxTv-lFy0IKBTiq_IdGXnjJVLYYohY8Qx-MqNw2wxVWa8c5iPtXc7rIqVObgQS7WKJi_VoZxtRJMKO3mT9qayYcw45ipsKrzL0Vj0fvYmVgdMznpMz8mTjfEJX5z3S_L9w_tv15_qmy8fP19f3dQWqMx1V77BkaFgFDrZ8ZZ2TA1rUANXBqkZ-la0SgLtgLNCAmws51z0vBdWSMMvSX16N_3CaV7rKbq9iUcdjNPnq105oQYGksvCq3_ypTXDH9G9kHGqFFAQRfv6pC3gzxlT1nuXlp-bEcOcNBMgu5YpWGzghNoYUoq4eTBiVC_B6q0-B6uXYDVtdQm26F6dLeb1HocH1X2SBXh3ArA09eAw6mQdjhYHF9FmPQT3X4u3f71gvRudNX6HR0zbMMexJKaZTkWgvy7TtQwXgzJXAnr-G2zMzes</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Leo, Giuseppina</creator><creator>Guescini, Michele</creator><creator>Genedani, Susanna</creator><creator>Stocchi, Vilberto</creator><creator>Carone, Chiara</creator><creator>Filaferro, Monica</creator><creator>Sisti, Davide</creator><creator>Marcoli, Manuela</creator><creator>Maura, Guido</creator><creator>Cortelli, Pietro</creator><creator>Guidolin, Diego</creator><creator>Fuxe, Kjell</creator><creator>Agnati, Luigi Francesco</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>20150401</creationdate><title>Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles</title><author>Leo, Giuseppina ; 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In these patients, β-adrenergic receptor function was altered. This prompted us to investigate whether the β-adrenergic receptor agonist isoproterenol, at a dose that stimulates peripheral β-adrenergic system but has no effects at the central nervous system, can induce anxiety-like behavior in rats. Moreover, some possible messengers involved in the peripheral to brain communication were investigated. Our results showed that isoproterenol (5 mg kg − 1 i.p.) increased heart rate, evoked anxiety-like behavior, did not result in motor impairments and increased extracellular vesicle content in the blood. Plasma corticosterone level was unmodified as well as vesicular Hsp70 content. Vesicular miR-208 was also unmodified indicating a source of increased extracellular vesicles different from cardiomyocytes. We can hypothesize that peripheral extracellular vesicles might contribute to the β-adrenergic receptor-evoked anxiety-like behavior, acting as peripheral signals in modulating the mental state.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>25654993</pmid><doi>10.1016/j.physbeh.2015.02.002</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Anxiety - blood Anxiety - chemically induced Anxiety-like behavior Blood Chemical Analysis Blotting, Western Cardiovascular Agents - pharmacology Corticosterone - blood Electrocardiography Extracellular vesicles Extracellular Vesicles - drug effects Extracellular Vesicles - metabolism Heart Rate - drug effects Hsp70 HSP70 Heat-Shock Proteins - metabolism Isoproterenol Isoproterenol - pharmacology Male Medicin och hälsovetenskap MicroRNAs - metabolism miR-208 Motor Activity - drug effects Psychiatry Psychotropic Drugs - pharmacology Rats, Sprague-Dawley |
| title | Acute isoproterenol induces anxiety-like behavior in rats and increases plasma content of extracellular vesicles |
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