Exploring the microbiome of healthy and diseased peri‐implant sites using Illumina sequencing

Aim To compare the microbiome of healthy (H) and diseased (P) peri‐implant sites and determine the core peri‐implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMINGS. Differences between groups were determined using principal coordinate analysis (PCoA), t tests and Wilcoxon rank sum test and FDR‐adjusted. The peri‐implant core microbiome was determined. Results PCoA showed partitioning between H and P at all taxonomic levels. Bacteroidetes, Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H (p