Gα i2 Signaling Regulates Inflammasome Priming and Cytokine Production by Biasing Macrophage Phenotype Determination
Macrophages exist as innate immune subsets that exhibit phenotypic heterogeneity and functional plasticity. Their phenotypes are dictated by inputs from the tissue microenvironment. G-protein-coupled receptors are essential in transducing signals from the microenvironment, and heterotrimeric Gα sign...
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Veröffentlicht in: | The Journal of immunology (1950) 2019-03, Vol.202 (5), p.1510-1520 |
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Sprache: | eng |
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Zusammenfassung: | Macrophages exist as innate immune subsets that exhibit phenotypic heterogeneity and functional plasticity. Their phenotypes are dictated by inputs from the tissue microenvironment. G-protein-coupled receptors are essential in transducing signals from the microenvironment, and heterotrimeric Gα signaling links these receptors to downstream effectors. Several Gα
-coupled G-protein-coupled receptors have been implicated in macrophage polarization. In this study, we use genetically modified mice to investigate the role of Gα
on inflammasome activity and macrophage polarization. We report that Gα
in murine bone marrow-derived macrophages (BMDMs) regulates IL-1β release after activation of the NLRP3, AIM2, and NLRC4 inflammasomes. We show this regulation stems from the biased polarity of Gα
deficient (
) and RGS-insensitive Gα
(
) BMDMs. We determined that although
BMDMs (excess Gα
signaling) have a tendency toward classically activated proinflammatory (M1) phenotype,
BMDMs (Gα
deficient) are biased toward alternatively activated anti-inflammatory (M2) phenotype. Finally, we find that Gα
-deficient macrophages have increased Akt activation and IFN-β production but defects in ERK1/2 and STAT3 activation after LPS stimulation. Gα
-deficient macrophages also exhibit increased STAT6 activation after IL-4 stimulation. In summary, our data indicates that excess Gα
signaling promotes an M1 macrophage phenotype, whereas Gα
signaling deficiency promotes an M2 phenotype. Understanding Gα
-mediated effects on macrophage polarization may bring to light insights regarding disease pathogenesis and the reprogramming of macrophages for the development of novel therapeutics. |
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ISSN: | 0022-1767 1550-6606 |
DOI: | 10.4049/jimmunol.1801145 |