Identification of a discrete subpopulation of spinal cord ependymal cells with neural stem cell properties

Spinal cord ependymal cells display neural stem cell properties in vitro and generate scar-forming astrocytes and remyelinating oligodendrocytes after injury. We report that ependymal cells are functionally heterogeneous and identify a small subpopulation (8% of ependymal cells and 0.1% of all cells...

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Veröffentlicht in:Cell reports (Cambridge) 2022-03, Vol.38 (9), p.110440-110440, Article 110440
Hauptverfasser: Stenudd, Moa, Sabelström, Hanna, Llorens-Bobadilla, Enric, Zamboni, Margherita, Blom, Hans, Brismar, Hjalmar, Zhang, Shupei, Basak, Onur, Clevers, Hans, Göritz, Christian, Barnabé-Heider, Fanie, Frisén, Jonas
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Sprache:eng
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Zusammenfassung:Spinal cord ependymal cells display neural stem cell properties in vitro and generate scar-forming astrocytes and remyelinating oligodendrocytes after injury. We report that ependymal cells are functionally heterogeneous and identify a small subpopulation (8% of ependymal cells and 0.1% of all cells in a spinal cord segment), which we denote ependymal A (EpA) cells, that accounts for the in vitro stem cell potential in the adult spinal cord. After spinal cord injury, EpA cells undergo self-renewing cell division as they give rise to differentiated progeny. Single-cell transcriptome analysis revealed a loss of ependymal cell gene expression programs as EpA cells gained signaling entropy and dedifferentiated to a stem-cell-like transcriptional state after an injury. We conclude that EpA cells are highly differentiated cells that can revert to a stem cell state and constitute a therapeutic target for spinal cord repair. [Display omitted] •Troy expression defines a subpopulation of spinal cord ependymal (EpA) cells•EpA cells harbor the in vitro stem cell capacity of the adult spinal cord•EpA cells dedifferentiate and acquire stem cell properties after spinal cord injury•EpA cells self-renew while generating astrocytes and oligodendrocytes after injury Stenudd et al. identify a subpopulation of ependymal cells defined by Troy expression, EpA cells, that contains the in vitro stem cell capacity of the spinal cord. These cells dedifferentiate to self-renewing stem cells after spinal cord injury and generate scar-forming astrocytes as well as oligodendrocytes.
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2022.110440