The Nodewalk assay to quantitate chromatin fiber interactomes in very small cell populations
The chromosome conformation capture method and its derivatives, such as circularized chromosome conformation capture, carbon copy chromosome conformation capture, high-throughput chromosome conformation capture and capture high-throughput chromosome conformation capture, have pioneered our understan...
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Veröffentlicht in: | Nature protocols 2023-03, Vol.18 (3), p.755-782 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The chromosome conformation capture method and its derivatives, such as circularized chromosome conformation capture, carbon copy chromosome conformation capture, high-throughput chromosome conformation capture and capture high-throughput chromosome conformation capture, have pioneered our understanding of the principles of chromosome folding in the nucleus. These technical advances, however, cannot precisely quantitate interaction frequency in very small input samples. Here we describe a protocol for the Nodewalk assay, which is based on converting chromosome conformation capture DNA samples to RNA and subsequently to cDNA using strategically placed primers. This pipeline enables the quantitative analyses of chromatin fiber interactions without compromising its sensitivity down to |
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ISSN: | 1754-2189 1750-2799 1750-2799 |
DOI: | 10.1038/s41596-022-00774-8 |