High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5–200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses. Lipid nanoparticles, viruses, exosomes and liposomes are characterized by analysis of fluorescence fluctuations.