β-Adrenergic Receptor Function in Cultured AT-1 Cardiomyocytes

β-Adrenergic Receptor Function in Cultured AT-1 Cardiomyocytes

AT-1 cells are highly differentiated, contracting cardiomyocytes derived from atrial tumours in transgenic mice. The aim of this study was to characterize β-adrenergic receptor function and associated intracellular calcium regulation in AT-1 cells. Equilibrium binding experiments with [ 3H]-CGP-1217...

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Veröffentlicht in:Biochemical and biophysical research communications 1995, Vol.207 (1), p.13-19
Hauptverfasser: Drvota, V., Wei, H., Haggblad, J., Carlsson, B., Sylven, C.
Format: Artikel
Sprache:eng
Schlagworte:
Adrenergic beta-Antagonists - metabolism
Alprenolol - pharmacology
Animals
Calcium - metabolism
Cell Line
Cyclic AMP - metabolism
Cytosol - metabolism
Heart - drug effects
Heart Neoplasms
Isoproterenol - pharmacology
Kinetics
Medicin och hälsovetenskap
Mice
Mice, Transgenic
Myocardial Contraction
Myocardium - metabolism
Potassium - pharmacology
Propanolamines - metabolism
Receptors, Adrenergic, beta - metabolism
Receptors, Adrenergic, beta - physiology
Time Factors
Tumor Cells, Cultured
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Zusammenfassung:AT-1 cells are highly differentiated, contracting cardiomyocytes derived from atrial tumours in transgenic mice. The aim of this study was to characterize β-adrenergic receptor function and associated intracellular calcium regulation in AT-1 cells. Equilibrium binding experiments with [ 3H]-CGP-12177 showed a K d=0.30±0.08nM and a B max=2.25±0.47 fmol/10 5cells. Competition binding experiments with CGP-20712A showed presence of predominantly β 1-adrenoreceptors. S-(−)propranolol, atenolol and R-(+)propranolol showed a competitive inhibition of binding with successively lower affinity. Isoproterenol, 2 μM, for 48 hours down-regulated the number (p
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1995.1146