Analysis of sodium dodecyl sulfate-stable cell wall aminopeptidases in strains of viridans streptococci

Abstract Cell wall extracts from nine strains of viridans streptococci representing five species were analyzed for aminopeptidases. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains, all havin...

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Veröffentlicht in:	FEMS microbiology letters 1996-09, Vol.143 (1), p.19-23
Hauptverfasser:	Linder, Lars E., Lönnies, Helena, Sund, Marie-louise
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminopeptidase Aminopeptidases - analysis Aminopeptidases - immunology Aminopeptidases - isolation & purification Arginine aminopeptidase Bacteriology Biological and medical sciences Cell surface Cell wall Cell Wall - enzymology Cell walls Chromatography, Gel Cross Reactions Crossed immunoelectrophoresis Electrophoresis Electrophoresis, Polyacrylamide Gel Enzyme Stability Fundamental and applied biological sciences. Psychology Gel electrophoresis Humans Immunoelectrophoresis Immunoelectrophoresis, Two-Dimensional Medicin och hälsovetenskap Metabolism. Enzymes Metalloprotease Microbiology Molecular Weight Mouth - microbiology Polyacrylamide Sodium Sodium Dodecyl Sulfate Sodium lauryl sulfate Species Specificity Streptococci Streptococcus Streptococcus - classification Streptococcus - enzymology Streptococcus mitis Streptococcus mutans Streptococcus salivarius Streptococcus sanguis Sulfates Viridans streptococci
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Zusammenfassung:	Abstract Cell wall extracts from nine strains of viridans streptococci representing five species were analyzed for aminopeptidases. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis in combination with zymogram procedures revealed a common pattern of aminopeptidases in six strains, all having enzyme bands at 89 and 45 kDa. Three strains had an additional band at 200 kDa. Crossreactivity between aminopeptidases of all active strains was shown with crossed immunoelectrophoresis. Strains of Streptococcus mutans and S. sobrinus were without detectable cell wall aminopeptidases.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1996.tb08455.x