An Approach to Monitor Exocytosis in White Adipocytes

Exocytosis, the fusion of vesicles with the plasma membrane, can be measured with the patch-clamp technique as increases in membrane capacitance. Here we provide detailed information on how to monitor white adipocyte exocytosis using this method. We describe how to isolate the stromal vascular fract...

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Veröffentlicht in:	Exocytosis and Endocytosis. Florence Niedergang, Nicolas Vitale, Stéphane Gasman (red.) Nicolas Vitale, Stéphane Gasman (red.), 2021, Vol.2233, p.203-222
Hauptverfasser:	Komai, Ali M, Shrestha, Man Mohan, Musovic, Saliha, Olofsson, Charlotta S
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3-L1 adipocytes 3T3-L1 cell line 3T3-L1 Cells Adipocyte differentiation Adipocytes, White - metabolism Adipocytes, White - ultrastructure Animals cell fractionation Cell isolation Cell Membrane - genetics Cell Membrane - ultrastructure Endocrinology and Diabetes Endokrinologi och diabetes Exocytosis Exocytosis - genetics human cell Membrane capacitance Mice patch clamp technique Patch-clamp Patch-Clamp Techniques - methods Stromal vascular cell fraction White adipocyte whole cell Whole-cell configuration
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Zusammenfassung:	Exocytosis, the fusion of vesicles with the plasma membrane, can be measured with the patch-clamp technique as increases in membrane capacitance. Here we provide detailed information on how to monitor white adipocyte exocytosis using this method. We describe how to isolate the stromal vascular fraction of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.
ISSN:	1064-3745 1940-6029
DOI:	10.1007/978-1-0716-1044-2_14