Snd3 controls nucleus-vacuole junctions in response to glucose signaling

Membrane contact sites facilitate the exchange of metabolites between organelles to support interorganellar communication. The nucleus-vacuole junctions (NVJs) establish physical contact between the perinuclear endoplasmic reticulum (ER) and the vacuole. Although the NVJ tethers are known, how NVJ abundance and composition are controlled in response to metabolic cues remains elusive. Here, we identify the ER protein Snd3 as central factor for NVJ formation. Snd3 interacts with NVJ tethers, supports their targeting to the contacts, and is essential for NVJ formation. Upon glucose exhaustion, Snd3 relocalizes from the ER to NVJs and promotes contact expansion regulated by central glucose signaling pathways. Glucose replenishment induces the rapid dissociation of Snd3 from the NVJs, preceding the slow disassembly of the junctions. In sum, this study identifies a key factor required for formation and regulation of NVJs and provides a paradigm for metabolic control of membrane contact sites. [Display omitted] •Snd3 is essential for the formation of nucleus-vacuole junctions•Snd3 links structural changes of nucleus-vacuole junctions to glucose availability•Glucose exhaustion triggers the targeting of Snd3 to the junctions•Snd3 promotes contact site expansion downstream of PKA and Snf1 signaling Membrane contact sites connect virtually all organelles within a cell and serve as metabolic hubs. Tosal-Castano et al. establish Snd3 as a key factor required for the formation and regulation of the contact sites between the nucleus and the vacuole in response to central glucose signaling pathways.