Vagococcus elongatus sp. nov., isolated from a swine-manure storage pit

1 Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA 2 Culture Collection, Department of Clinical Bacteriology, University of Göteborg, S-41346 Göteborg, Sweden 3 Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, USA Correspondence Terence R. Whitehead whitehtr{at}ncaur.usda.gov A polyphasic taxonomic study was performed on an uncharacterized Gram-positive, catalase-negative, elongated coccus-shaped bacterium isolated from a swine-manure storage pit. The bacterium, designated strain PPC9 T , was facultatively anaerobic and had a DNA G+C content of 44.5 mol%. Comparative 16S rRNA gene sequencing indicated that the bacterium represented a novel subline within the genus Vagococcus , close to but distinct from Vagococcus lutrae . Strain PPC9 T was readily distinguished from the five recognized species of the genus Vagococcus by using biochemical tests and molecular genetic analysis. Based on phylogenetic and phenotypic evidence strain PPC9 T is considered to represent a novel species of the genus Vagococcus , for which the name Vagococcus elongatus sp. nov. is proposed. The type strain is PPC9 T (=CCUG 51432 T =NRRL B-41357 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain PPC9 T is AF445297 .