Biomarkers of disease and post-mortem changes — Heat stabilization, a necessary tool for measurement of protein regulation

This review focuses on post sampling changes and how the Stabilizor system has been used to control this natural biological process and potential implications on cancer-specific biomarkers due to post sampling changes. Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level [1] resulting in loss of sample quality due to post-mortem changes. A heat-stabilization technology, using the Stabilizor system, has been developed to quickly and permanently abolish the enzymatic activity that causes these changes post-sampling and so preserve sample quality. The Stabilizor system has been shown to give better sample quality when analyzing a variety of tissues in various proteomic workflows. In this paper we discuss the impact of using heat-stabilized tissue in different proteomic applications. Based on our observations regarding the overlap between commonly changing proteins and proteins found to change post-mortem we also highlight a group of proteins of particular interest in cancer studies. ► The uses of the Stabilizor system is reviewed. ► Multiple proteomic investigations benefit from heat stabilization. ► Heat stabilization enables study of endogenous peptides. ► Tissue proteomics with high sample quality. ► Several biomarkes linked to cancer are severely effected by post-sampling changes.