Anthocyanin Characterization Utilizing Liquid Chromatography Combined with Advanced Mass Spectrometric Detection

Anthocyanins are naturally occurring polyphenolic plant pigments. To analyze the anthocyanin content of samples, rapid and reliable methods for separation and detection are needed. In this work an LC−DAD−ESI-MS/MS instrument was used to develop a new tandem MS data acquisition strategy for anthocyanin characterization which was subsequently compared to more conventional measurements. It has been shown that the newly developed strategy, multiple reaction monitoring-initiated anthocyanin characterization (MIAC), can successfully be used in anthocyanin analysis and has various advantages compared to some more traditional measurements, such as enhanced selectivity, better signal-to-noise ratio and simplified data evaluation. Furthermore, the number of relevant MS/MS data increased significantly with the MIAC method compared with a more common information dependent MS experiment strategy.